A Copper Single‐Atom Cascade Bionanocatalyst for Treating Multidrug‐Resistant Bacterial Diabetic Ulcer

Diabetic ulcers induced by multidrug-resistant (MDR) bacteria have severely endangered diabetic populations. These are very challenging to treat because the local high glucose concentration can both promote bacterial growth and limit immune system's bactericidal action. Herein, a oxidase-peroxidase (GOx-POD) dual-enzyme mimetic (DEM) bionanocatalyst, [email protected] is synthesized simultaneously control in ulcers. Specifically, AuNPs serve as GOx mimics catalyze oxidation of for formation H2O2; H2O2 then be further catalytically converted into OH via POD-mimetic copper single atoms. Notably, unique atoms coordinated one oxygen two nitrogen (CuN2O1) exhibit better POD catalytic performance than natural peroxidase. Further DFT calculations conducted study mechanism reveal advantage this CuN2O1 structure compared other single-atom sites. Both vitro vivo experiments confirm outstanding antibacterial therapeutic efficacy DEM bionanocatalyst. This new bionanocatalyst will provide essential insights next generation antibiotic-free strategies combating MDR ulcers, also offer inspiration designing bionanocatalytic cascading medicines.